Review



attenuated vaccine strain rm48  (Novogene)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Novogene attenuated vaccine strain rm48
    The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the <t>RM48</t> strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).
    Attenuated Vaccine Strain Rm48, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/attenuated vaccine strain rm48/product/Novogene
    Average 86 stars, based on 1 article reviews
    attenuated vaccine strain rm48 - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains"

    Article Title: A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains

    Journal: Transboundary and Emerging Diseases

    doi: 10.1155/tbed/5823134

    The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the RM48 strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).
    Figure Legend Snippet: The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the RM48 strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).

    Techniques Used: Concentration Assay, Amplification

    Sensitivity validation of the RNase H2‐linked differential qPCR assay. (A) Amplification curves of the standard plasmid pUC57‐vaccine at serial concentrations: 1 × 10 8 copies/µL, 1 × 10 7 copies/µL, 1 × 10 6 copies/µL, 1 × 10 5 copies/µL, 1 × 10 4 copies/µL, 1 × 10 3 copies/µL, 1 × 10 2 copies/µL, 10 copies/µL and 1 copy/µL. (B) Standard curve of the pUC57‐vaccine plasmid, plotted as log 10 copies/µL versus cycle threshold (Ct) value. (C) Amplification curves of the live‐attenuated vaccine strain 168‐L genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (D) Standard curve of strain 168‐L, plotted as log 10 CCU 50 versus Ct value. (E) Amplification curves of the live‐attenuated vaccine strain RM48 genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (F) Standard curve of strain RM48, plotted as log 10 CCU 50 versus Ct value.
    Figure Legend Snippet: Sensitivity validation of the RNase H2‐linked differential qPCR assay. (A) Amplification curves of the standard plasmid pUC57‐vaccine at serial concentrations: 1 × 10 8 copies/µL, 1 × 10 7 copies/µL, 1 × 10 6 copies/µL, 1 × 10 5 copies/µL, 1 × 10 4 copies/µL, 1 × 10 3 copies/µL, 1 × 10 2 copies/µL, 10 copies/µL and 1 copy/µL. (B) Standard curve of the pUC57‐vaccine plasmid, plotted as log 10 copies/µL versus cycle threshold (Ct) value. (C) Amplification curves of the live‐attenuated vaccine strain 168‐L genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (D) Standard curve of strain 168‐L, plotted as log 10 CCU 50 versus Ct value. (E) Amplification curves of the live‐attenuated vaccine strain RM48 genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (F) Standard curve of strain RM48, plotted as log 10 CCU 50 versus Ct value.

    Techniques Used: Biomarker Discovery, Amplification, Plasmid Preparation

    Repeatability validation of the RNase H2‐linked qPCR assay: Ct value distribution and coefficient of variation (CV) for the standard plasmid pUC57‐vaccine, 168‐L, and RM48 (10 technical replicates per template).
    Figure Legend Snippet: Repeatability validation of the RNase H2‐linked qPCR assay: Ct value distribution and coefficient of variation (CV) for the standard plasmid pUC57‐vaccine, 168‐L, and RM48 (10 technical replicates per template).

    Techniques Used: Biomarker Discovery, Plasmid Preparation



    Similar Products

    attenuated vaccine strain rm48  (Novogene)
    86
    Novogene attenuated vaccine strain rm48
    The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the <t>RM48</t> strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).
    Attenuated Vaccine Strain Rm48, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/attenuated vaccine strain rm48/product/Novogene
    Average 86 stars, based on 1 article reviews
    attenuated vaccine strain rm48 - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the RM48 strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).

    Journal: Transboundary and Emerging Diseases

    Article Title: A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains

    doi: 10.1155/tbed/5823134

    Figure Lengend Snippet: The optimization results of the RNase H2‐linked qPCR assay. (A) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the 168‐L strain. (B) Probe concentration gradient (0.4/0.45/0.5/0.55/0.6 µM) for the RM48 strain. (C) Evaluation of nonspecific amplification of the wild‐type strain 168 under different probe concentrations (0.4/0.45/0.5/0.55/0.6 µM). (D) Annealing temperature gradient (58/59/60/61/63°C) for the 168‐L strain. (E) Annealing temperature gradient (58/59/60/61/63°C) for the RM48 strain. (F) Evaluation of nonspecific amplification of the wild‐type strain 168 under different annealing temperatures (58/59/60/61/63°C).

    Article Snippet: Then, specific primers (Supporting Information Table ) for the selected candidate targets were designed to amplify the live‐attenuated vaccine strain RM48, and the amplification products were sent to Novogene Co., Ltd. for sequencing.

    Techniques: Concentration Assay, Amplification

    Sensitivity validation of the RNase H2‐linked differential qPCR assay. (A) Amplification curves of the standard plasmid pUC57‐vaccine at serial concentrations: 1 × 10 8 copies/µL, 1 × 10 7 copies/µL, 1 × 10 6 copies/µL, 1 × 10 5 copies/µL, 1 × 10 4 copies/µL, 1 × 10 3 copies/µL, 1 × 10 2 copies/µL, 10 copies/µL and 1 copy/µL. (B) Standard curve of the pUC57‐vaccine plasmid, plotted as log 10 copies/µL versus cycle threshold (Ct) value. (C) Amplification curves of the live‐attenuated vaccine strain 168‐L genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (D) Standard curve of strain 168‐L, plotted as log 10 CCU 50 versus Ct value. (E) Amplification curves of the live‐attenuated vaccine strain RM48 genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (F) Standard curve of strain RM48, plotted as log 10 CCU 50 versus Ct value.

    Journal: Transboundary and Emerging Diseases

    Article Title: A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains

    doi: 10.1155/tbed/5823134

    Figure Lengend Snippet: Sensitivity validation of the RNase H2‐linked differential qPCR assay. (A) Amplification curves of the standard plasmid pUC57‐vaccine at serial concentrations: 1 × 10 8 copies/µL, 1 × 10 7 copies/µL, 1 × 10 6 copies/µL, 1 × 10 5 copies/µL, 1 × 10 4 copies/µL, 1 × 10 3 copies/µL, 1 × 10 2 copies/µL, 10 copies/µL and 1 copy/µL. (B) Standard curve of the pUC57‐vaccine plasmid, plotted as log 10 copies/µL versus cycle threshold (Ct) value. (C) Amplification curves of the live‐attenuated vaccine strain 168‐L genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (D) Standard curve of strain 168‐L, plotted as log 10 CCU 50 versus Ct value. (E) Amplification curves of the live‐attenuated vaccine strain RM48 genomic DNA at serial dilutions: 10 0.5 CCU 50 /mL, 10 1.5 CCU 50 /mL, 10 2.5 CCU 50 /mL, 10 3.5 CCU 50 /mL, 10 4.5 CCU 50 /mL, 10 5.5 CCU 50 /mL, 10 6.5 CCU 50 /mL, and 10 7.5 CCU 50 /mL. (F) Standard curve of strain RM48, plotted as log 10 CCU 50 versus Ct value.

    Article Snippet: Then, specific primers (Supporting Information Table ) for the selected candidate targets were designed to amplify the live‐attenuated vaccine strain RM48, and the amplification products were sent to Novogene Co., Ltd. for sequencing.

    Techniques: Biomarker Discovery, Amplification, Plasmid Preparation

    Repeatability validation of the RNase H2‐linked qPCR assay: Ct value distribution and coefficient of variation (CV) for the standard plasmid pUC57‐vaccine, 168‐L, and RM48 (10 technical replicates per template).

    Journal: Transboundary and Emerging Diseases

    Article Title: A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains

    doi: 10.1155/tbed/5823134

    Figure Lengend Snippet: Repeatability validation of the RNase H2‐linked qPCR assay: Ct value distribution and coefficient of variation (CV) for the standard plasmid pUC57‐vaccine, 168‐L, and RM48 (10 technical replicates per template).

    Article Snippet: Then, specific primers (Supporting Information Table ) for the selected candidate targets were designed to amplify the live‐attenuated vaccine strain RM48, and the amplification products were sent to Novogene Co., Ltd. for sequencing.

    Techniques: Biomarker Discovery, Plasmid Preparation